The draw was published by the Football Association this lunchtime and sees clubs from Enterprise National League North and South enter the competition.
The match will be played on Saturday 15 November, kick off 15:00.
Admission prices and ticket information will be announced soon.
The Isuzu FA Trophy, the premier knockout competition for Step 1-4 clubs in the National League System, was introduced in 1969. The current holders are Aldershot Town, who beat Spennymore Town 3-0.
Isuzu FA Trophy second round draw
1 FC United of Manchester v Darlington
2 Chester v Clitheroe
3 Macclesfield v Prescot Cables
4 Gainsborough Trinity v Alfreton Town
5 Chorley v Worksop Town
6 Leek Town v Scarborough Athletic
7 Radcliffe v Bradford (Park Avenue)
8 Buxton v Southport
9 Spennymoor Town v AFC Fylde
10 South Shields v Dunston
11 Marine v Stocksbridge Park Steels
12 Ilkeston Town v Curzon Ashton
13 King’s Lynn Town v Harrow Borough
14 Merthyr Town v Hanwell Town
15 Slough Town v Gloucester City
16 Kidderminster Harriers v Corby Town
17 Bedford Town v Anstey Nomads
18 Chesham United v AFC Telford United
19 Brentwood Town v Hornchurch
20 AFC Sudbury v Hereford
21 Leamington v Maidenhead United
22 Oxford City v Peterborough Sports
23 Bishop’s Stortford v Cheshunt
24 Hemel Hempstead Town v Chelmsford City
25 Alvechurch v Real Bedford
26 Harborough Town v Enfield Town
27 Dagenham & Redbridge v Tilbury
28 Walton & Hersham v Farnborough
29 Horsham v Farnham Town
30 Dover Athletic v Basingstoke Town or Dorchester Town
31 Torquay United v Maidstone United
32 Chippenham Town v Weston Super Mare
33 Wimborne Town v Chatham Town
34 Plymouth Parkway v Salisbury
35 Bath City v Bracknell Town
36 Ebbsfleet United v Sittingbourne
37 Folkestone Invicta v AFC Totton
38 Dorking Wanderers v Eastbourne Borough
39 Tonbridge Angels v Worthing
40 Leatherhead v Hampton & Richmond Borough
2025/26 Isuzu FA Trophy round dates
Second Round: Saturday 15 November 2025
Third Round: Saturday 13 December 2025
Fourth Round: Saturday 10 January 2026
Fifth Round: Saturday 31 January 2026
Sixth Round: Saturday 28 February 2026
Semi-Final: Saturday 28 March 2026
The Final: Sunday 17 May 2026
2025/26 Isuzu FA Trophy prize fund
Second Round: Winners receive £3750, losers receive £1000
Third Round: Winners receive £4500, losers receive £1250
Fourth Round: Winners receive £5250, losers receive £1500
Fifth Round: Winners receive £6000, losers receive £1750
Sixth Round: Winners receive £7500, losers receive £2000
Semi-Final: Winners receive £15,000, losers receive £5000
Final runners-up: £30,000
Final winners: £60,000
